Study on the molecular mechanism of endostatin and doxycycline in suppressing melanoma growth / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 677-680, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-268868
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular mechanism of endostatin and doxycycline effect on melanoma growth.</p><p><b>METHODS</b>A B16 melanoma mice model was established by intracutaneous injection of B16 cell suspension. The mice were treated with endostatin, doxycycline, endostatin and doxycycline respectively, the control group received no treatment. A time course study of tumor volume was performed to observe the antitumor effect. The expression of matrix metalloproteinase (MMP-9), MMP-2, TIMP-2 were examined by immunohistochemistry staining.</p><p><b>RESULTS</b>Tumors in endostatin treatment group, doxycycline treatment group, endostatin and doxycycline treatment group grew slower than in the control group. The difference of the average tumor volume in the doxycycline group and control group, in the doxycycline with endostatin treatment group and control group were statistically different. The positive expression ratio of MMP-2, MMP-9, TIMP-2 in each treatment group were statistically different from their control groups (F = 12.79, F = 5.56, F = 4.64; P < 0.05).</p><p><b>CONCLUSION</b>Doxycycline and endostatin are able to inhibit the expression of MMPs and promote expression of TIMP, which ultimately inhibits the growth of B16 melonoma.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Melanoma Experimental
/
Inmunohistoquímica
/
Doxiciclina
/
Inhibidor Tisular de Metaloproteinasa-2
/
Metaloproteinasa 2 de la Matriz
/
Metaloproteinasa 9 de la Matriz
/
Metaloproteinasas de la Matriz
/
Línea Celular Tumoral
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Pathology
Año:
2006
Tipo del documento:
Artículo
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