Role of K(Ca)3.1 channel in proliferation and migration of rat vascular smooth muscle cells of the proliferative phenotype / 南方医科大学学报
Journal of Southern Medical University
;
(12): 976-980, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-268951
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of K(Ca)3.1 channel in the proliferation and migration of rat vascular smooth muscle cells of the proliferative phenotype.</p><p><b>METHODS</b>Rat vascular smooth muscle cells (VSMCs) were cultured with tissue adhesion method. The morphological characteristics of the fist and ninth passages of VSMCs were observed with light and electron microscopy and immunocytochemistry. The expressions of K(Ca)3.1 channel mRNA and protein in the cells were detected using RT-PCR and immunocytochemistry, respectively. MTT and transwell assay were employed to assess the effect of the K(Ca)3.1 channel blocker TRAM-34 on the proliferation and migration of VSMCs.</p><p><b>RESULTS</b>The first and ninth passages of VSMCs showed morphological characteristics of contractile and proliferative phenotypes, respectively. Compared with the first- passage cells, the ninth-passage VSMCs exhibited significantly increased K(Ca)3.1 channel mRNA and protein expressions with enhanced cell proliferation and migration (P<0.01), which was inhibited by the application of TRAM-34 (P<0.01). TRAM-34 produced no obvious effect on the first-passage VSMCs.</p><p><b>CONCLUSION</b>Upregulated expression of K(Ca)3.1 channel can promote the proliferation and migration of rat VSMCs of the proliferative phenotype.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Pirazoles
/
Movimiento Celular
/
Células Cultivadas
/
Ratas Sprague-Dawley
/
Biología Celular
/
Bloqueadores de los Canales de Potasio
/
Miocitos del Músculo Liso
/
Proliferación Celular
/
Canales de Potasio de Gran Conductancia Activados por el Calcio
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2012
Tipo del documento:
Artículo
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