Effect of hepatitis C virus core gene transfection on NFAT1 expression in human intrahepatic cholangiocarcinoma cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 789-793, 2012.
Article
en Zh
| WPRIM
| ID: wpr-268997
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether hepatitis C virus core protein (HCV C) regulates the expression of NFAT1 to participate in the progression and malignant biological behavior of intrahepatic cholangiocarcinoma cells.</p><p><b>METHODS</b>The recombinant plasmid pEGFP-N(3)-HCV C and the empty vector pEGFP-N(3) were cotransfected with enhanced green fluorescent protein (EGFP) into RBE cells using liposome. Real-time PCR and Western blotting were used to examine the expression of NFAT1 mRNA and protein in the transfected RBE cells. MTT assay was used to evaluate the changes in the cell proliferation, and the cell cycle changes were analyzed by flow cytometry.</p><p><b>RESULTS</b>HCV C transfection significantly enhanced the expressions of NFAT1 mRNA and protein in RBE cells (P<0.05) and promoted the progression of cell cycle into G(2)/M phase to accelerate the cell proliferation.</p><p><b>CONCLUSION</b>Transfection with HCV C gene up-regulates NFAT1 expression and promotes the cell cycle progression and proliferation of intrahepatic cholangiocarcinoma cells, suggesting the involvement of HCV C in the progression of intrahepatic cholangiocarcinoma.</p>
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Índice:
WPRIM
Asunto principal:
Patología
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Plásmidos
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Neoplasias de los Conductos Biliares
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Transfección
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Expresión Génica
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Ciclo Celular
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Proteínas del Núcleo Viral
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Colangiocarcinoma
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Línea Celular Tumoral
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Proliferación Celular
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2012
Tipo del documento:
Article