Effect of saikosaponin-d on rat's glomerular mesangial cell proliferation and extracellular matrix hyperplasia in vitro / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 321-325, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-269069
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of saikosaponin-d (SSd) on glomerular mesangial cells (MCs) proliferation and hyperplastic extracellular matrix (ECM) induced by lipopolysaccharide (LPS) to provide experimental proof for its use in prevention and treatment of glomerulosclerosis.</p><p><b>METHODS</b>Rat's MCs were cultivated and identified. The cultured MCs were stimulated by LPS and incubated with different concentrations of SSd. Cell proliferation was determined by MTT assay, LDH assay and flow cytometry, respectively. Type IV collagen (Col IV), fibronectin (FN) and transforming growth factor beta1 (TGF-beta1) in the conditioned medium were measured by ELISA. The expressions of cyclin-dependent kinase 4 (CDK4), c-Jun and c-Fos were detected by immunohistochemistry.</p><p><b>RESULTS</b>After treated by SSd, MC proliferation was inhibited, cells in G0/G1 phase increased, and apoptosis induced. Moreover, secretion of Col IV, FN and TGF-beta1 and the expressions of CDK4, c-Jun and c-Fos in MC were inhibited.</p><p><b>CONCLUSION</b>The inhibitory action of SSd on glomerulosclerosis was realized through inhibiting the expressions of CDK4, c-J un and c-Fos.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ácido Oleanólico
/
Patología
/
Farmacología
/
Saponinas
/
Ensayo de Inmunoadsorción Enzimática
/
Inmunohistoquímica
/
Ciclo Celular
/
Supervivencia Celular
/
Células Cultivadas
/
Lipopolisacáridos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2007
Tipo del documento:
Artículo
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