DAPT enhances the apoptosis of human tongue carcinoma cells / 国际口腔科学杂志·英文版
International Journal of Oral Science
; (4): 81-89, 2009.
Article
en En
| WPRIM
| ID: wpr-269731
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the effect of DAPT (gamma-secretase inhibitor) on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma.</p><p><b>METHODOLOGY</b>Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growth was determined using Indigotic Reduction method. The cell cycle and apoptosis were analyzed by flow cytometry. Real-time PCR and Immuno-Fluorescence (IF) were employed to determine the intracellular expression levels.</p><p><b>RESULTS</b>DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G0-G1 cell cycle arrest and apoptosis. The mRNA levels of Hairy/Enhancer of Split-1 (Hes-1), a target of Notch activation, were reduced by DAPT in a dose-dependent manner. Coincident with this observation, DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells.</p><p><b>CONCLUSION</b>DAPT may have a therapeutic value for human tongue carcinoma. Moreover, the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch-1 and Caspase-3.</p>
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Índice:
WPRIM
Asunto principal:
Patología
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Farmacología
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Proteínas Represoras
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Neoplasias de la Lengua
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Carcinoma
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Membrana Celular
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Núcleo Celular
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Fase de Descanso del Ciclo Celular
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Fase G1
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Apoptosis
Límite:
Humans
Idioma:
En
Revista:
International Journal of Oral Science
Año:
2009
Tipo del documento:
Article