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DAPT enhances the apoptosis of human tongue carcinoma cells / 国际口腔科学杂志·英文版
Article en En | WPRIM | ID: wpr-269731
Biblioteca responsable: WPRO
ABSTRACT
<p><b>AIM</b>To investigate the effect of DAPT (gamma-secretase inhibitor) on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma.</p><p><b>METHODOLOGY</b>Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growth was determined using Indigotic Reduction method. The cell cycle and apoptosis were analyzed by flow cytometry. Real-time PCR and Immuno-Fluorescence (IF) were employed to determine the intracellular expression levels.</p><p><b>RESULTS</b>DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G0-G1 cell cycle arrest and apoptosis. The mRNA levels of Hairy/Enhancer of Split-1 (Hes-1), a target of Notch activation, were reduced by DAPT in a dose-dependent manner. Coincident with this observation, DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells.</p><p><b>CONCLUSION</b>DAPT may have a therapeutic value for human tongue carcinoma. Moreover, the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch-1 and Caspase-3.</p>
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Patología / Farmacología / Proteínas Represoras / Neoplasias de la Lengua / Carcinoma / Membrana Celular / Núcleo Celular / Fase de Descanso del Ciclo Celular / Fase G1 / Apoptosis Límite: Humans Idioma: En Revista: International Journal of Oral Science Año: 2009 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Patología / Farmacología / Proteínas Represoras / Neoplasias de la Lengua / Carcinoma / Membrana Celular / Núcleo Celular / Fase de Descanso del Ciclo Celular / Fase G1 / Apoptosis Límite: Humans Idioma: En Revista: International Journal of Oral Science Año: 2009 Tipo del documento: Article