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Preliminary study of ALK3 downstream genes related to ventricular septum defect / 生物工程学报
Chinese Journal of Biotechnology ; (12): 267-271, 2003.
Artículo en Chino | WPRIM | ID: wpr-270071
ABSTRACT
To investigate the function of ALK3 gene, the gene regulation and the signaling pathway related to ventricular septum defect during heart development. The model mice with ALK3 gene knock-out via alpha-MHC-Cre/lox P system were bred. The mRNA expression level of control group was compared with that of experiment group and ALK3 downstream genes were screened using PCR-select cDNA subtraction microarray. The mRNA of control group was extracted from E11.5 normal mouse hearts, and that of experiment group, from E11.5 hearts of mice with alpha-MHC Cre(+/-) ALK3(F/+) genotype. It was found that the mice with ALK3 gene knock-out produced heart defects involving the interventricular septum. The platelet-activating factors acetylhydrolase and the transcription factor Pax-8 and so on, were down-regulated. However, the Protein Tyrosine Kinase (PTK) of Focal Adhesion Kinase (FAK) subfamily and beta subtype protein 14-3-3 were up-regulated in the alpha-MHC Cre(+/-) ALK3(F/-) mice. These data provide support that ALK3 gene played an important role during heart development. The platelet-activating factors acetylhydrolase and Pax-8 genes could be important ALK3 downstream genes in the BMP signaling pathway during interventricular septum development. PTK and beta subtype protein 14-3-3 might be regulatory factors in this pathway.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Proteínas Tirosina Quinasas / Transducción de Señal / Ratones Noqueados / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Análisis de Secuencia por Matrices de Oligonucleótidos / 1-Alquil-2-acetilglicerofosfocolina Esterasa / Proteínas 14-3-3 / Receptores de Proteínas Morfogenéticas Óseas de Tipo 1 / Factores de Transcripción Paired Box Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Proteínas Tirosina Quinasas / Transducción de Señal / Ratones Noqueados / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Análisis de Secuencia por Matrices de Oligonucleótidos / 1-Alquil-2-acetilglicerofosfocolina Esterasa / Proteínas 14-3-3 / Receptores de Proteínas Morfogenéticas Óseas de Tipo 1 / Factores de Transcripción Paired Box Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2003 Tipo del documento: Artículo