Biosynthesis of poly (3-mercaptopropionate) and poly (3-mercaptopropionate-co-3-hydroxybutyrate) with recombinant Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 195-199, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-270114
ABSTRACT
Polythioesters newly emerged as a type of novel polymer and they have showed great potential for application in industries. In this study, genes of butyrate kinase (buk) and phosphotransbutyrylase (ptb) from Clostridium acetobutylicum, and poly (3-hydroxybutyrate) (PHB) synthase gene from Thiocapsa pfennigii were used for construction of a metabolic pathway to synthesize the polythioesters. When 3-mercaptopropionate and 3-hydroxybutyrate were fed, poly (3-mercaptopropoinate) [poly (3MP)] and poly(3-mercaptopropionate-co-3-hydroxybutyrate) [poly(3MP-co-3HB)] were synthesized by recombinant Escherichia coli JM109 (pBPP1) harboring the constructed metabolic pathway. Results indicated clearly that all these genes are necessary for the synthesis of poly(3MP) and poly(3MP-co-3HB).
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosfato Acetiltransferasa
/
Plásmidos
/
Polímeros
/
Espectrofotometría Infrarroja
/
Aciltransferasas
/
Química
/
Fosfotransferasas (aceptor de Grupo Carboxilo)
/
Ácido 3-Hidroxibutírico
/
Escherichia coli
/
Genética
Idioma:
Inglés
Revista:
Chinese Journal of Biotechnology
Año:
2003
Tipo del documento:
Artículo
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