Construction and identification of Ksp-cadherin-Gpx1-Klk1 expression vector / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1327-1330, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-270149
ABSTRACT
<p><b>OBJECTIVE</b>To construct a Gpx1 and klk1 recombinant vector containing the kidney-specific promoter Ksp-cadherin.</p><p><b>METHODS</b>Human Gpx1, Klk1 and Ksp-cadherin cDNAs were amplified with PCR and inserted in a stepwise manner into the expressive vector pIRES-EGFP to construct the recombinant vector Ksp-cadherin-Gpx1-Klk1. The constructed vector was verified with restriction enzyme digestion and sequence analysis.</p><p><b>RESULTS AND CONCLUSION</b>The recombinant expression vector Ksp-cadherin-Gpx1-Klk1 was constructed and identified successfully, which provides a potent tool for preparing transgenic animals to investigate gene therapy for ischemia-reperfusion injury in kidney transplantation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Calicreínas
/
Terapia Genética
/
Cadherinas
/
Regiones Promotoras Genéticas
/
Clonación Molecular
/
Vectores Genéticos
/
Genética
/
Glutatión Peroxidasa
/
Riñón
/
Metabolismo
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2008
Tipo del documento:
Artículo
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