Cloning of Rcet3, a novel gene related to family 2 cystatins / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1151-1153, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-270189
ABSTRACT
<p><b>OBJECTIVE</b>To clone the full-length Rcet3 gene, a novel gene related to family 2 cystatins, from mouse testis or other tissues.</p><p><b>METHODS</b>Rcet3 gene was cloned using digital differential display (DDD) and RT-PCR was performed for cloning the full-length Rcet3 gene from adult mouse testis cDNA library with sequence analysis.</p><p><b>RESULTS</b>Rcet3 cDNA was 610 bp in length, consisting of 4 exons to encode a protein with 140 amino acid residues. The encoded protein contained a potential signal peptide and a cystatin domain, but lacked critical consensus site important for cysteine protease inhibition. These characteristics could be seen in the Cres subgroup related to the family 2 cystatins. Rcet3 was specifically expressed in adult mouse testis, epididymis and the cerebrum, but at higher levels in the testis than in the epididymis and cerebrum.</p><p><b>CONCLUSION</b>Rcet3 may be a new member of Cres subgroup of family 2 cystatins.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Testículo
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Cistatinas
/
Química
/
Secuencia de Aminoácidos
/
Clonación Molecular
/
Análisis de Secuencia de ADN
/
ADN Complementario
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2008
Tipo del documento:
Artículo
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