Effect of basic fibroblast growth factor and hyaluronic acid on proliferation of rabbit chondrocytes in vitro / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 42-44, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-270282
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of basic fibroblast growth factor (bFGF) and hyaluronic acid (HA) on the proliferation of rabbit chondrocytes in vitro.</p><p><b>METHODS</b>Chondrocytes from the knee joints of New Zealand white rabbits were cultured. bFGF or HA or both were added into the culture medium respectively, and the proliferation of the chondrocytes was measured with MTT 3-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyl-tetra-zolium bromide. (MTT, Sigma, M2128).</p><p><b>RESULTS</b>Basic fibroblast growth factor (10 ng/ml) with low concentration of fetal bovine serum in the culture medium promoted the proliferation of chondrocytes significantly, and this effect reached its maximum when concentration of bFGF reached 50 ng/ml. HA itself had no effect on the proliferation of chondrocytes. However, when bFGF was used in combination with HA, especially when the concentration of bFGF was 50-500 ng/ml and that of HA was 10-50 ng/ml, the effect on the proliferation of chondrocytes was much more than when bFGF or HA was used alone.</p><p><b>CONCLUSIONS</b>bFGF can promote the proliferation of chondrocytes. HA, which has no effect on the proliferation of the cells, can maintain a normal growth of chondrocytes. When bFGF is used in combination with HA, more proliferation is obtained.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fisiología
/
Técnicas In Vitro
/
División Celular
/
Células Cultivadas
/
Probabilidad
/
Análisis de Varianza
/
Factor 2 de Crecimiento de Fibroblastos
/
Sensibilidad y Especificidad
/
Condrocitos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Journal of Traumatology
Año:
2004
Tipo del documento:
Artículo
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