Effects of basic fibroblast growth factor on biological characteristics of osteoblasts / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 229-233, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-270325
ABSTRACT
<p><b>OBJECTIVE</b>To elucidate the effects of exogenous basic fibroblast growth factor (bFGF) on biological characteristics of rat osteoblasts cultured in vitro.</p><p><b>METHODS</b>The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-beta(1)) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts.</p><p><b>RESULTS</b>bFGF (5-50 ng/ml) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-beta(1) mRNA increased significantly, but the intracellular ALP content decreased.</p><p><b>CONCLUSIONS</b>bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-beta(1), but cannot promote the differentiation of osteoblasts.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Farmacología
/
ARN Mensajero
/
Células Cultivadas
/
Factor 2 de Crecimiento de Fibroblastos
/
Factor de Crecimiento Transformador beta
/
Ratas Sprague-Dawley
/
Antígeno Nuclear de Célula en Proliferación
/
Fosfatasa Alcalina
/
Factor de Crecimiento Transformador beta1
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Journal of Traumatology
Año:
2003
Tipo del documento:
Artículo
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