Application of pulse-field gel electrophoresis analysis in source-tracking of food-borne disease caused by Vibrio parahaemolyticus / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 36-38, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-270461
ABSTRACT
<p><b>OBJECTIVE</b>To apply pulse-field gel electrophoresis analysis(PFGE) in analysing a case of food poisoning caused by Vibrio parahaemolyticus.</p><p><b>METHODS</b>PFGE using restriction enzyme Not I was employed in molecular subtyping of thirty strains of V. parahaemolyticus isolated from a case of food poisoning in Guangzhou city and PFGE patterns were analyzed by using BioNumerics Version 4.0 software to perform cluster analysis. Pattern profiles were compared by using the Dice coefficient and unweighted pair group method with arithmetic averages (UPGMA).</p><p><b>RESULTS</b>Thirty strains were of the same type of pulsotype.</p><p><b>CONCLUSIONS</b>Molecular subtyping by PFGE might disclose the epidemiological relationships of the strains from humans, food and the environment, giving a strong molecular epidemiological evidence and a support for the source-tracking of outbreak events.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Vibrio parahaemolyticus
/
China
/
Técnicas de Tipificación Bacteriana
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Clasificación
/
Electroforesis en Gel de Campo Pulsado
/
Enfermedades Transmitidas por los Alimentos
/
Genética
/
Métodos
/
Microbiología
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Preventive Medicine
Año:
2008
Tipo del documento:
Artículo
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