Rapid Identification of Bacterial Species Associated with Bronchiectasis via Metagenomic Approach / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 898-901, 2014.
Article
en En
| WPRIM
| ID: wpr-270525
Biblioteca responsable:
WPRO
ABSTRACT
Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved. However, 30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria, making it difficult to confirm the pathogen. In this study, we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation, and sent for 16S rDNA pyrosequencing by a 454 GS Junior machine. Metagenomic analysis showed the composition of bacterial community in sample was complex. More than a half of reads (51.3%) were from Pseudomonas aeruginosa. This result was corresponding with the culture result but came out 2 d earlier, which is meaningful for early diagnosis and treatment. The detection with 16S rDNA pyrosequencing technology is more sensitive and rapid than routine culture, and can detect the co-infection or symbiosis in airway, giving us a novel and convenient approach to perform rapid diagnosis.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Pseudomonas aeruginosa
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Infecciones por Pseudomonas
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Factores de Tiempo
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Bronquiectasia
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Líquido del Lavado Bronquioalveolar
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ARN Ribosómico 16S
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Química
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Diagnóstico Precoz
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Metagenoma
/
Metagenómica
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Año:
2014
Tipo del documento:
Article