Induction of apoptosis in hormone-resistant human prostate cancer PC3 cells by inactivated Sendai virus / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 506-514, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-270572
ABSTRACT
<p><b>OBJECTIVE</b>Inactivated Sendai virus particle [hemagglutinating virus of Japan envelope (HVJ-E)] has a potential oncolytic effect due to its ability to induce apoptosis in tumor cells. However, the molecular mechanism of apoptosis induction in cancer cells mediated by HVJ-E has not been fully elucidated. This paper aims to investigate the underlying mechanism of apoptosis induction by HVJ-E in prostate cancer cells (PC3).</p><p><b>METHODS</b>PC3 cells were treated with HVJ-E at various MOI, and then interferon-β (IFN-β) production, and the cell viability and apoptosis were detected by ELISA, MTT-based assay and flow cytometry, respectively. Next, the roles of Jak-Stat, MAPK and Akt pathways played in HVJ-E-induced apoptosis in PC3 cells were analyzed by immunoblot assay. To further evaluate the cytotoxic effect of HVJ-E on PC3 cells, HVJ-E was intratumorally injected into prostate cancers on BALB/c-nude mice, and the tumor volume was monitored for 36 days.</p><p><b>RESULTS</b>HVJ-E induced IFN-β production and activated Jak-Stat signaling pathway, which resulted in the activation of caspase-8, caspase-3, and PARP in PC3 prostate cancer cells post HVJ-E treatment. Furthermore, we observed for the first time that p38 and Jnk MAPKs in PC3 cells contributed to HVJ-E-induced apoptosis. In addition, intratumoral HVJ-E treatment displayed a direct inhibitory effect in an in vivo BALB/c nude mouse prostate cancer model.</p><p><b>CONCLUSION</b>Our findings have provided novel insights into the underlying mechanisms by which HVJ-E induces apoptosis in tumor cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Neoplasias de la Próstata
/
Vacunas de Productos Inactivados
/
Regulación Neoplásica de la Expresión Génica
/
Apoptosis
/
Vacunas contra el Cáncer
/
Virus Sendai
/
Línea Celular Tumoral
/
Alergia e Inmunología
/
Viroterapia Oncolítica
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2014
Tipo del documento:
Artículo
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