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Effect of sterilization methods on cell binding activity of surface-immobilized fibronectin / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 391-395, 2014.
Artículo en Inglés | WPRIM | ID: wpr-270589
ABSTRACT
The effect of sterilization methods on biological activity of fibronectin on the surface of biomaterials was elaborated in the present study. Sterile protein- modified biomaterials were fabricated by microfilter filtration and UV irradiation, respectively. UV irradiation altered the conformation of surface- adsorbed fibronectin and further affected the attachment, morphology and biological function of endothelial cells. However, microfilter filtration did not to change the normal conformation of fibronectin, or the proliferation and biological function of endothelial cells, indicating that microfilter filtration sterilization is the most suitable method for protein-substrate.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Prótesis e Implantes / Efectos de la Radiación / Rayos Ultravioleta / Adhesión Celular / Esterilización / Fibronectinas / Filtración / Métodos / Microbiología Idioma: Inglés Revista: Biomedical and Environmental Sciences Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Prótesis e Implantes / Efectos de la Radiación / Rayos Ultravioleta / Adhesión Celular / Esterilización / Fibronectinas / Filtración / Métodos / Microbiología Idioma: Inglés Revista: Biomedical and Environmental Sciences Año: 2014 Tipo del documento: Artículo