Quantitative (stereological) study of incomplete spermatogenic suppression induced by testosterone undecanoate injection in rats / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 291-297, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-270892
ABSTRACT
<p><b>AIM</b>To evaluate the key lesions in spermatogenesis suppressed partially by testosterone undecanoate (TU) treatment.</p><p><b>METHODS</b>Adult male SD rats were treated with vehicle or TU (19 mg/kg) injection (i.m.) every 15 days for 130 days. The numbers of all types of cells (nuclei) in the seminiferous tubules and the interstitial tissue were estimated using a contemporary stereological tool, the optical disector.</p><p><b>RESULTS</b>In response to TU treatment, the numbers of non-type B spermatogonia, type B spermatogonia and late elongated spermatids per testis were reduced to 51 %, 66 % and 14 % of the controls, respectively. The conversion ratios from type B spermatogonia to early spermatocytes and pachytene spermatocytes were not significantly affected and the ratios to the later germ cell types fell to 51 % - 65 % of the controls. Less than 1.0 % of immature round spermatids were seen sloughing into the tubule lumen, 4.0 % of elongated spermatids retained in the seminiferous epithelium, and about half of the elongated spermatid nuclei appreciably malformed. Leydig cells were atrophied but their number and the peritubular myoid cell number per testis were unchanged.</p><p><b>CONCLUSION</b>Double inhibition of spermatogenesis (i.e. inhibition at spermiation and spermatogonial conversion to type B spermatogonia), a scenario seen in the monkey and human following gonadotrophin withdrawal, was not sufficiently effective for a complete spermatogenic suppression in the rat after TU treatment, probably due to ineffective inhibition of the Leydig cell population and therefore the intra-testicular testosterone levels.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Células de Sertoli
/
Recuento de Espermatozoides
/
Espermátides
/
Espermatogénesis
/
Testosterona
/
Sangre
/
Núcleo Celular
/
Ratas Sprague-Dawley
/
Depresión Química
Límite:
Animales
Idioma:
Inglés
Revista:
Asian Journal of Andrology
Año:
2004
Tipo del documento:
Artículo
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