Identification of differentially expressed genes involved in multidrug resistance in K562/A02 by cDNA microarray / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 328-332, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-271021
ABSTRACT
<p><b>OBJECTIVE</b>To study the molecular mechanism underlying multidrug resistance (MDR) and identify unknown genes that might be involved in drug resistance development in K562/A02 cells.</p><p><b>METHODS</b>K562/A02 was induced by gradually increasing the ADM concentration in culture medium of K562 cells, the differential expression of associated genes between K562 and K562/A02 was determined with cDNA microarray. Overexpression of neurofilament protein NF-H gene in K562/A02 cells was confirmed with RT-PCR and immunocytochemistry. Anti-sense oligodeoxynucleotides were transfected into K562/A02 cells by lipofectamine in order to further analyze the role of NF-H in drug resistance.</p><p><b>RESULTS</b>Comparing with the expression profiles, we found upregulation of 5 transcripts and downregulation of 7 transcripts in response to MDR of K562/A02 cells. The overexpression of NF-H, one of the 5 upregulated genes, was confirmed. After being treated with antisense oligodeoxynucleotides of NF-H and mdr1, the cellular adriamycin concentration increased significantly, but antisense NF-H alone did not have significant effect on drug resistance phenotype.</p><p><b>CONCLUSION</b>The development of MDR in K562/A02 cells is multifactorial. NF-H may be involved in the drug resistance of K562/A02, which may provide a new marker of diagnosis and a new target of therapy.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
ARN Mensajero
/
Doxorrubicina
/
Regulación hacia Arriba
/
Proteínas de Neurofilamentos
/
Resistencia a Múltiples Medicamentos
/
Resistencia a Antineoplásicos
/
Genes MDR
/
Células K562
/
Oligodesoxirribonucleótidos Antisentido
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2004
Tipo del documento:
Artículo
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