Construction of adenovirus vector expressing TIP30 and its tumor suppressive effect in vitro and in vivo / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 85-88, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-271060
ABSTRACT
<p><b>OBJECTIVE</b>To construct an adenovirus vector expressing TIP30 gene (Ad-TIP30) and investigate its tumor suppressive effect in vitro and in vivo.</p><p><b>METHODS</b>Ad-Easy system was used to construct Ad-TIP30 by recombination in E. coli. The virus was packaged in 293 cells and subsequently identified valid. Human HCC (hepatocellular carcinoma) cell lines HepG(2) (p53-wt), PLC/PRL/5 (p53-mut), and osteosarcoma cell line Saos-2 (p53-null) with different p53 genotype were infected with Ad-TIP30 and control virus with Ad-GFP, respectively. The tumor suppressive effect of TIP30 in vitro was examined by trypan blue exclusion method. The expression level of p53 was determined by RT-PCR before and after Ad-TIP30 infection. The in vivo tumor suppressive effect was detected in nude mice with human HCC xenograft.</p><p><b>RESULTS</b>The expression of TIP30 significantly inhibited the in vitro proliferation of tumor cells, among which HepG(2) with wild type p53 gene was most susceptible to Ad-TIP30 induced growth inhibition. The expression of p53 was significantly up-regulated in HepG(2) after Ad-TIP30 infection as determined by RT-PCR. The growth in nude mice of HCC infected with Ad-TIP30 was significantly inhibited with an inhibition rate of 62.9%.</p><p><b>CONCLUSION</b>The expression of TIP30 could inhibit the proliferation of tumor cell lines through both p53-dependent and p53-independent pathways, and may be used as a potential tool for cancer therapy.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Acetiltransferasas
/
Terapéutica
/
Factores de Transcripción
/
Terapia Genética
/
División Celular
/
Adenoviridae
/
Genes p53
/
Vectores Genéticos
/
Genética
/
Neoplasias Experimentales
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2004
Tipo del documento:
Artículo
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