Antisense DNMT1 gene fragment in the sensitivity change of SMMC-7721 cells to tumor necrosis factor related apoptosis inducing ligand and its mechanism / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 538-541, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-271086
ABSTRACT
<p><b>OBJECTIVE</b>To observe the sensitivity change of SMMC-7721 cells transfected with antisense DNMT1 gene fragment to tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its mechanism.</p><p><b>METHODS</b>Cell survival rate was measured by trypan blue, apoptosis rate by TUNEL method and the expression of bcl-2, bax and bad by flow cytometry.</p><p><b>RESULTS</b>Cell survival rate of SMMC-7721 cells transfected with antisense DNMT1 gene fragment was markedly lower than that transfected with sense DNMT1 gene fragment or empty vector (P < 0.05 and 0.01), but the apoptosis rate was on the contrary (P < 0.05 or 0.01). The expression of bax and bad (especially the former), but not bcl-2 of SMMC-7721 cells transfected with antisense DNMT1 gene fragment was markedly higher than those of SMMC-7721 cells transfected with sense DNMT1 gene fragment or empty vector.</p><p><b>CONCLUSION</b>The sensitivity of SMMC-7721 cells to TRAIL can be enhanced by the transfection of antisense DNMT1 gene fragment, which may be related to the increase of bax and bad expression.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Glicoproteínas de Membrana
/
Proteínas Portadoras
/
Elementos sin Sentido (Genética)
/
Proteínas Proto-Oncogénicas
/
Factor de Necrosis Tumoral alfa
/
Apoptosis
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Línea Celular Tumoral
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2003
Tipo del documento:
Artículo
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