Rapid purification of human albumin with immunomagnetic microsphere technology / 药学学报
Acta Pharmaceutica Sinica
;
(12): 608-614, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-271399
ABSTRACT
<p><b>AIM</b>To develop a rapid and inexpensive method for purification of human albumin, a method of immunomagnetic microspheres (IMMS) based on enzyme-linked immunosorbent assay (ELISA) for the purification of human albumin from human serum.</p><p><b>METHODS</b>Polystyrene magnetic microspheres with carboxyl groups as carriers were prepared, and then the carboxyl groups on the surface of the microspheres were activated by ethylcarbodiimide (EDC). Finally rabbit anti-human serum albumin (HSA) antibodies were covalently bound to it and the complex can specifically capture HSA. After the procedure of capturing HSA, through taking rabbit anti-human albumin protein antibodies as a capture antibody, and goat anti-human albumin protein antibodies as a detection antibody, an ELISA on IMMS was developed, which can determine the recovery yield of HSA from the human serum.</p><p><b>RESULTS</b>The result of the experiment was that the recovery of human albumin with IMMS was (86 +/- 4)%, and IMMS were reused for two other purifying cycles, the results of which were (69.0 +/- 0.6)% and (40.8 +/- 0.8)%, and the purity of the product was about 90%.</p><p><b>CONCLUSION</b>The results above prove that the immunomagnetic purifiying strategy was shown to be efficient and offers an new thought for a large scale production of high-purity HSA.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Poliestirenos
/
Albúmina Sérica
/
Ensayo de Inmunoadsorción Enzimática
/
Química
/
Reproducibilidad de los Resultados
/
Separación Inmunomagnética
/
Alergia e Inmunología
/
Métodos
/
Microesferas
Límite:
Humanos
Idioma:
Inglés
Revista:
Acta Pharmaceutica Sinica
Año:
2006
Tipo del documento:
Artículo
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