Adhesional inhibiton of polyclonal anti-porin I antibody / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 78-83, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-271572
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the blockness effects of purified polyclonal anti-porin I antibody on N. gonorrhoeae adherence to genitourinary tract epithelia of BALB/c mouse.</p><p><b>METHODS</b>Polyclonal anti GST-PI antibody was generated by immunizing rabbit with GST-PI fusion protein which was constructed and expressed by ourselves. The purified immunoglobulin G was obtained by ammonium sulphate deposition and DEAE cellulose chromatography. Mice model of gonorrhea was established. In order to evaluate the effects of PI-IgG on gonococcus adhesion to vagina mucus, the macroscopic and pathological assessing as well as gonococcus culture was employed after gonococcus challenge on PI-IgG immunized mice.</p><p><b>RESULT</b>No pus and pathological inflammation were observed on mice vagina mucus treated with 1 mg/ml PI-IgG 3 hours before gonococcus challenge. Gonococcus could not be detected in the smears and washing solutions from vagina. Pathological inflammation was found in mice treated with anti PI-IgG, in which the concentrations were lower than 1 mg/ml or the treated time was longer than 3 hours prior to gonococcus challenge.</p><p><b>CONCLUSION</b>The purified anti PI-IgG can effectively inhibit the adherence and infection of gonococci to genitourinary tract epithelia of BALB/c mice. In addition, the blocking duration of anti PI-IgG is associated with antibody concentration.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fisiología
/
Sistema Urogenital
/
Adhesión Bacteriana
/
Proteínas Recombinantes de Fusión
/
Gonorrea
/
Porinas
/
Alergia e Inmunología
/
Epitelio
/
Genética
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2007
Tipo del documento:
Artículo
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