Cardioprotective effect of ischemic postconditioning and preconditioning against prolonged ischemia and reperfusion induced injury in isolated rat heart / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 35-40, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-271579
ABSTRACT
<p><b>OBJECTIVE</b>To explore the cardioprotection effect of co-treatment with ischemic postconditioning and preconditioning in ischemia/reperfusion (I/R) injury and the related mechanism.</p><p><b>METHODS</b>Male Sprague-Dawley rats were used for Langendorff isolated heart perfusion. The hearts were subjected to global ischemia for 60 min followed by 120 min of reperfusion. The cardiomyocyte viability was measured by MTT-formazan method, and the cardiac injury was evaluated by the levels of lactate dehydrogenase (LDH) in the coronary effluent. Ventricular hemodynamic parameters were also measured.</p><p><b>RESULT</b>In 60 min of ischemia and 120 min of reperfusion group, ischemic postconditioning increased formazan content, reduced LDH release, but hemodynamic parameters did not improved. Co-treatment with ischemic postconditioning and preconditioning during the prolonged ischemia further improved the hemodynamic parameters. The calcium activated potassium channel antagonist paxilline attenuated the effect of co-treatment with ischemic postconditioning and preconditioning.</p><p><b>CONCLUSION</b>Ischemic postconditioning and preconditioning may synergically protect myocardium from severe ischemia injury, which may be related to calcium-activated potassium channel.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Técnicas In Vitro
/
Daño por Reperfusión Miocárdica
/
Supervivencia Celular
/
Ratas Sprague-Dawley
/
Isquemia Miocárdica
/
Precondicionamiento Isquémico Miocárdico
/
Canales de Potasio Calcio-Activados
/
L-Lactato Deshidrogenasa
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2007
Tipo del documento:
Artículo
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