Stimulation by Lycium bararum polysaccharides of the maturation of dendritic cells in murine bone marrow / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 648-652, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-271592
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Lycium bararum polysaccharides (LBPs) stimulation on the maturation of murine bone marrow derived dendritic cells (BMDCs).</p><p><b>METHODS</b>Murine bone marrow cells were cultured in GM-CSF and IL-4 for 5 days, then were purified with a MACS column. Respectively, BMDCs were stimulated with LBPs, LPS and RPMI1640 for 2 days. Cell phenotypes and antigens uptake by BMDCs were analyzed by flow cytometry. Cytokines released by BMDCs were detected. The antigen presenting by BMDCs was evaluated by mixed lymphocyte responses.</p><p><b>RESULT</b>Compared with to the BMDCs that only subjected to RPMI 1640, the expression of I-A/I-E, CD11c and secretion of IL-12 by BMDCs stimulated with LBPs were increased, the phagocytosis of FITC-dextran by BMDCs stimulated with LBPs was impaired but the activation of proliferation of allogenic lymphocytes by BMDCs was strengthened.</p><p><b>CONCLUSION</b>LBPs promote not only the maturation of cultured murine BMDCs in vitro, but also the immune response initiation induced by BMDCs.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fagocitosis
/
Farmacología
/
Células Dendríticas
/
Células de la Médula Ósea
/
Medicamentos Herbarios Chinos
/
Linfocitos
/
Células Cultivadas
/
Lipopolisacáridos
/
Prueba de Cultivo Mixto de Linfocitos
/
Biología Celular
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2006
Tipo del documento:
Artículo
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