HPLC determination of two flavonoid compounds in Psoralea corylifolia / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 669-671, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-271811
ABSTRACT
<p><b>OBJECTIVE</b>To determine two flavonoid compounds in Psoralea corylifolia L. (PC) simultaneously with HPLC method.</p><p><b>METHOD</b>Bavachin and corylin isolated from PC and purified in our laboratory were used as the reference compounds. The HPLC separation was carried out on an Techsphere ODS column using mobile phase consisting of a mixture of methanol and 20 mmol.L-1 ammonium acetate buffer pH 4.0 (6733), and the UV detection wavelength was 240 nm.</p><p><b>RESULT</b>Simultaneous determination of bavachin and corylin was achieved. The linear range was 1.25-20 micrograms.mL-1 for both bavachin and corylin. The average recovery of bavachin and corylin was 94.9% and 96.2%, and RSD was 3.1% and 3.6% respectively.</p><p><b>CONCLUSION</b>This is the first report on simultaneous determination of bavachin and corylin in PC with satisfactory accuracy and reproducibility.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas Medicinales
/
Flavonoides
/
Química
/
Cromatografía Líquida de Alta Presión
/
Psoralea
/
Frutas
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2002
Tipo del documento:
Artículo
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