Effect of NF-κB activation on the radiation response of esophageal cancer cells / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 485-488, 2014.
Article
en Zh
| WPRIM
| ID: wpr-272349
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of NF-κB activation on radiation response of esophageal carcinoma.</p><p><b>METHODS</b>The expression of NF-κB was detected in pretreatment and posttreatment specimens of patients with ESCC by immunohistochemistry. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activation of NF-κB in esophageal cancer cell line KYSE150 cells. SN50, a specific NF-κB inhibitor, was applied to inhibit the activation of NF-κB. Clone formation test was used to detect the radiosensitivity of esophageal cancer cells.</p><p><b>RESULTS</b>The median survival time of patients with activated and inactivated NF-κB in the pretreatment specimens were 16 and 19 months, respectively, with a non-significant difference between the two groups (P > 0.05). As to the patients with activated and inactivated NF-κB in posttreatment specimens, the median survival times were 13 and 35 months, respectively, with a significant difference (P < 0.01) between them. Western blot showed that the cytoplasmic expression of NF-κB was reduced with increasing radiation dose at 1.5 and 3 hours after radiation treatment. However, the expression of NF-κB in the cell nuclei was increased under the same condition, showing a trend of increased nucleus/cytoplasm ratio. The clone number in SN50 group was 96.66, 64.66, 76.66 and 10.00 under 0, 2, 4 and 12 Gy irradiation, which demonstrated a significant difference compared with the control groups (P < 0.001).</p><p><b>CONCLUSIONS</b>Our results show that activation of NF-κB is induced by radiotherapy. Activation of NF-κB reduces the outcome of radiation treatment of esophageal cancer patients.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Tolerancia a Radiación
/
Radioterapia
/
Neoplasias Esofágicas
/
Inmunohistoquímica
/
Transducción de Señal
/
Núcleo Celular
/
Western Blotting
/
FN-kappa B
/
Línea Celular Tumoral
/
Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Año:
2014
Tipo del documento:
Article