Influence of lipopolysaccharide on the permeability of rat brain microvascular endothelial cells and the molecular mechanism / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 908-911, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-272436
ABSTRACT
<p><b>OBJECTIVE</b>To study the influence of lipopolysaccharide (LPS) on the permeability of rat brain microvascular endothelial cells (BMECs) and possible molecular mechanism.</p><p><b>METHODS</b>Monolayers of primary rat BMECs were separated and cultured, and then treated with (LPS group) or without LPS (control group). The barrier integrity was measured by transendothelial electrical resistance (TEER) assay. The degrees of RhoA activation were determined by Pull-down assay. The expression levels of p115RhoGEF, zonula occludens-1 (ZO-1), occludin and claudin-5 proteins were detected by Western blot analysis.</p><p><b>RESULTS</b>The average TEER values of rat BMECs in the LPS group were 108.3±4.2 Ω•cm2 and 85.4±2.5 Ω•cm2 respectively 3 and 12 hrs after LPS treatment, which were significantly lower than that in the control group (159.0±8.6 Ω•cm2). Compared with the control group, the activity of RhoA started to increase 5 minutes after LPS treatment, and the expression of p115RhoGEF protein started to increase 1 hr after LPS treatment and the cellular protein levels of ZO-1, occludin and claudin-5 decreased significantly 3 hrs after LPS treatment in the LPS group (P<0.05).</p><p><b>CONCLUSIONS</b>LPS may activate the p115RhoGEF/RhoA pathway and decrease protein expression of ZO-1, occludin and claudin-5, resulting in an increased permeability of rat BMECs.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Encéfalo
/
Permeabilidad Capilar
/
Química
/
Lipopolisacáridos
/
Ratas Sprague-Dawley
/
Impedancia Eléctrica
/
Uniones Estrechas
/
Proteína de Unión al GTP rhoA
/
Factores de Intercambio de Guanina Nucleótido
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2011
Tipo del documento:
Artículo
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