Inductive Effect of 5-Azacitidine on Apoptosis of Multiple Myeloma Cell lines and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 110-116, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-272494
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the response of multiple myeloma (MM) cells to 5-azacitidine and its possible mechanisms.</p><p><b>METHODS</b>Two multiple myeloma-derived cell lines U266 and H929 were used in this study. The cell proliferation and apoptosis were assessed by CCK-8, flow cytometry and acridine orange staining. The cell cycle was assessed by flow cytometry. The expression of BCL-2, BAX was assessed by RT-PCR. The expressions of caspase-3 and p-ERK1/2 were assessed by Western blot.</p><p><b>RESULTS</b>The BCL-2/BAX ratio decreased, the activity of caspase-3 and p-ERK1/2 increased, the cell cycle was arrested in G2/M phase after treatment with 5-azacitidine. The 5-azacitidine inhibited proliferation in a dose-dependent manner.</p><p><b>CONCLUSION</b>5-azacitidine exerts apoptosis-inducing and grow-inhibiting effects on MM cell lines, its mechanism may be related with the decrease of BCL-2/BAX ratio, caspase-3 activation and the arrest of cell cycle.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Azacitidina
/
Ciclo Celular
/
Apoptosis
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Sistema de Señalización de MAP Quinasas
/
Línea Celular Tumoral
/
Proliferación Celular
/
Proteína X Asociada a bcl-2
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2016
Tipo del documento:
Artículo
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