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The effect of DNA methyltransferase 1 low expression on the global genome DNA methylation status of 16HBE cell / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 194-197, 2011.
Artículo en Chino | WPRIM | ID: wpr-272668
ABSTRACT
<p><b>OBJECTIVE</b>To construct DNA methyltransferase 1 (DNMT1) low expression 16HBE cell line and observe the variation of cell cycle and global genomic DNA methylation.</p><p><b>METHODS</b>The method of Lenti-virus induced RNA interference was applied to introduce four different shRNA fragment into 16HBE cells. Flow cytometry and 5-mC immunofluorescence methods were used to observe the cell cycle and global DNA methylation status of DNMT1 low expression 16HBE cells.</p><p><b>RESULTS</b>The DNMT1 protein relative expression level of 16HBE-shDNMT1-4 cell line was down regulated about 44% (P < 0.05) compared with the control. No obvious differences of cell cycle and global genome DNA methylation status were observed between the 16HBE and 16HBE-shDNMT1.</p><p><b>CONCLUSION</b>The DNMT1 gene low expression cell is successfully constructed, and there are no obvious changes happened on the cell cycle and global genomic DNA methylation.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Regulación hacia Abajo / Ciclo Celular / Línea Celular / Metilación de ADN / ARN Interferente Pequeño / Interferencia de ARN / ADN (Citosina-5-)-Metiltransferasas / Células Epiteliales / ADN (Citosina-5-)-Metiltransferasa 1 / Genética Límite: Humanos Idioma: Chino Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Regulación hacia Abajo / Ciclo Celular / Línea Celular / Metilación de ADN / ARN Interferente Pequeño / Interferencia de ARN / ADN (Citosina-5-)-Metiltransferasas / Células Epiteliales / ADN (Citosina-5-)-Metiltransferasa 1 / Genética Límite: Humanos Idioma: Chino Revista: Chinese Journal of Industrial Hygiene and Occupational Diseases Año: 2011 Tipo del documento: Artículo