Preparation and simultaneous determination of corynoline and acetylcorynoline in the herb of Corydalis bungeana / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 346-349, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-272858
ABSTRACT
<p><b>OBJECTIVE</b>To isolate and purify corynoline and acetylcorynoline from Corydalis bungeana and develop a reversed-phase HPLC method of determining the two components in C. bungeana.</p><p><b>METHOD</b>Alkaloids were isolated from the ethanolic extract with column gel chromatography, and identified on the basis of spectral analysis (UV, 1H-NMR, 13C-NMR) and physicochemical properties. For quantitative analysis of the two components, samples were separated on an ODS column with mobile phase of methanol-15 mmol.L-1 potassium dihydrogen phosphate/potassium phosphate dibasic (pH 6.70, 7030). The flow rate was 0.8 mL.min-1, and the detection was set at 289 nm.</p><p><b>RESULT</b>The purity was 99.5% and 99.1% for corynoline and acetylcorynoline respectively. The calibration curves were linear in the range of 6.9-110.4 mg.L-1 corynoline and 8.7-139.5 mg.L-1 acetylcorynoline. The RSD was 2.1% and 2.7%, and the average recovery was 97.3% and 97.2% respectively.</p><p><b>CONCLUSION</b>The method of isolating and purifying corynoline and acetylcorynoline from Corydalis bungeana and the HPLC method of simultaneous determination of the two components have been developed. The HPLC method is simple, easy to perform and applicable to the content determination of corynoline and acetylcorynoline in C. bungeana of various origins.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas Medicinales
/
Alcaloides de Berberina
/
Química
/
Cromatografía Líquida de Alta Presión
/
Corydalis
/
Métodos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2003
Tipo del documento:
Artículo
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