Study on preparation process and analytical methods of ESAC from Ganoderma lucidum / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 332-334, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-272864
ABSTRACT
<p><b>OBJECTIVE</b>To develop the procedure for separating the ethanol-soluble and acidic components (ESAC) from Ganoderma lucidum, and to establish a method for quantifying ESAC in G. lucidum.</p><p><b>METHOD</b>The ethanol extract of G. lucidum was extracted with a saturated NaHCO3 solution, acidified and re-extracted by chloroform to obtain ESAC. The quantitative analysis of ESAC was based on the characteristic color reaction between ESAC and H2SO4.</p><p><b>RESULT</b>The optimal conditions for separating ESAC on a 10 g scale are as follows ratio of material and ethanol (mL), 115; immersing time, 24 h; volume of saturated NaHCO3 and chloroform, 1300 mL; extract 3 times. The condition for measuring ESAC is as follows sample weight, 1 g; solution volume, 1.5 mL; immuersing time, 0.5 h; detecting reagent, 50% H2SO4 in ethanol; heating time in 100 degrees C water bathe, 3 min; measuring wavelength, 490 nm.</p><p><b>CONCLUSION</b>The procedure for ESAC preparation is simple and well-designed, and the established method for ESAC can be used for the qualitative analysis of the G. lucidum related products.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Triterpenos
/
Medicamentos Herbarios Chinos
/
Química
/
Tecnología Farmacéutica
/
Reishi
/
Etanol
/
Métodos
Tipo de estudio:
Investigación cualitativa
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2003
Tipo del documento:
Artículo
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