Tissue engineering of dentin-pulp complex-like structures by human dental mesenchymal cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 408-411, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-273201
ABSTRACT
<p><b>OBJECTIVE</b>To establish three-dimensional culture model of human dental mesenchymal cells and bioengineer in vivo with ceramic bovine bone (CBB) and Collagraft as scaffolds.</p><p><b>METHODS</b>Human dental mesenchymal cells induced upon stimulation of bFGF and IGF-1 or TGF-beta(1) were implanted onto CBB and Collagraft containing the same kinds of growth factors respectively. Then cell/scaffold constructs were transplanted into nude mice to establish in vivo culture model of dental mesenchymal cells. Control groups were set up at the same time. After 4 weeks or 10 weeks, the implants were taken out for histological and immunohistochemical analysis.</p><p><b>RESULTS</b>Within 10-week implant tissues, typical dentin-pulp complex-like structures were generated in scaffolds containing growth factors. Human dentin sialoprotein (DSP) was expressed in the newly formed dentin. This phenomenon wasn't observed in control groups and 4-week implants.</p><p><b>CONCLUSIONS</b>Dentin-pulp complex-like structures could be bioengineered successfully with human dental mesenchymal cells and CBB or Collagrafts containing growth factors in nude mice.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Diente Primario
/
Fosfatos de Calcio
/
Células Cultivadas
/
Embriología
/
Colágeno
/
Biología Celular
/
Ingeniería de Tejidos
/
Pulpa Dental
/
Dentina
/
Células Madre Mesenquimatosas
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2005
Tipo del documento:
Artículo
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