Identification and characterization of synovial mesenchymal stem cells in temporomandibular joint / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify and characterize mesenchymal stem cells from synovial membrane of temporomandibular joint in vitro.</p><p><b>METHODS</b>Synovial mesenchymal stem cells (SMSCs) were obtained by limited dilution method and expanded in 25 ml flasks. Methyl thiazolyl tetrazolium (MTT) method was used to determine the cell growth cycles. The expressions of vimentin and keratin were respectively detected with immunocytochemistry, while the expressions of CD8, CD34, CD44, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) were determined by flow cytometry.</p><p><b>RESULTS</b>Pure mesenchymal stem cells were of spindle shape and uniform in size, which were intensively positive in vimentin, but negative in keratin. The expression of CD44, VCAM-1 and ICAM-1 were also verified by flow cytometry.</p><p><b>CONCLUSIONS</b>Mesenchymal stem cells could be purified from adult synovial membrane of temporomandibular joint.</p>