Effect of respiratory syncytial virus on the activity of matrix metalloproteinase in mice / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 5-11, 2007.
Artículo
en Inglés
| WPRIM
| ID: wpr-273347
ABSTRACT
<p><b>BACKGROUND</b>Respiratory syncytial virus (RSV) is a common pathogen in the lower respiratory tract of infants and children. Recent studies have shown that in adults, especially in the elderly population who have relatively weak immunity, lower respiratory tract infection is not uncommon. RSV was detected in 22% hospitalized patients with acute exacerbation of chronic obstructive pulmonary disease (COPD), and the detection rate was only next to that of parvovirus and influenza virus respectively. Further studies revealed that lung infection of RSV could lead to inflammatory destruction and structural remodeling. This study was undertaken to examine the effect of infection with RSV on matrix metalloproteinase (MMPs) in mice, and to explore the role of RSV in the pathogenesis of pulmonary diseases.</p><p><b>METHODS</b>Twenty BALB/c mice were divided randomly into an RSV infection group and a blank control group. The mice in the RSV infection group were given 100 microl liquid containing 10(6) PFU of RSV by intranasal instillation. Three days after the infection, the bronchoalveolar lavage fluid (BALF) was harvested and RT-PCR and Western blotting were used to detect MMP-9 and the expression of tissue inhibitors of matrix metalloproteinase (TIMP)-1 mRNA in lung tissues. Gelatin zymography was employed to detect the activities of MMP-9 and MMP-2 in BALF. Immunohistochemistry was used to determine the expressions of E-cadherin (E-cd) and proliferating cell nuclear antigen (PCNA) in the lung tissues.</p><p><b>RESULTS</b>In the BALF of the mice infected with RSV, the activities of MMP-9 and MMP-2 were significantly increased (t = 6.08, P < 0.01 and t = 5.68, P < 0.01). The levels of mRNA and proteins of MMP-9 in the lung tissues of the mice infected with RSV were significantly elevated, and the mRNA and protein levels were significantly higher than those of the blank controls. Though the ratio of MMP-9/TIMP-1 mRNA in the lung tissues of the infected mice was not significantly different from that of the normal controls, the ratio of the MMP-9/TIMP-1 protein in the RSV infection group was significantly higher than that in the control group. Moreover, the number of cells with positive E-cd expression was decreased and the number of cells positive for PCNA was increased, with an enhanced expression.</p><p><b>CONCLUSIONS</b>In mice, infection with RSV can significantly increase the activities of MMP-9 and MMP-2, and conspicuously elevate the mRNA transcription of MMP-9. RSV infection can increase the activity of gelatinase while up-regulating its inhibitory factors but increase its protein ratio of MMP-9/TIMP-1 in lung tissues, thereby facilitating fibrosis after structural destruction of the airway. The resultant status might be an important factor causing chronic reconstruction of the airway.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virus Sincitiales Respiratorios
/
Virología
/
ARN Mensajero
/
Células HeLa
/
Cadherinas
/
Infecciones por Virus Sincitial Respiratorio
/
Antígeno Nuclear de Célula en Proliferación
/
Metaloproteinasa 2 de la Matriz
/
Metaloproteinasa 9 de la Matriz
/
Genética
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2007
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS