Screening study of the kinetogenic effects of serum containing four Chinese materia medicas on the colonic smooth muscle cells in rats / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 422-424, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-273681
ABSTRACT
<p><b>OBJECTIVE</b>To study the kinetogenic effects of serum containing Semen Arecae, Dandelion, Semen raphani and Atractylodes macrocephala on the colonic smooth muscle cells of rats.</p><p><b>METHODS</b>Serum containing Chinese materia medicas was made according to standard methods. Smooth muscle cells were isolated from the muscle layers of Wistar rat's colon, referred to modified Bitar's method. The contractile response of colonic smooth muscle cells to serum containing Chinese materia medicas (10%, 50%, 100% concentration) and other medicines (blank and 1 x 10(-3) mol/L acetylcholine) were separately observed. The contractility was presented by the decrease of the cell length between the drug groups and the control.</p><p><b>RESULTS</b>Serum containing each Chinese materia medica can make dose-dependent contraction at different concentrations (P < 0.05), but the strongest effect of each serum had no significant difference (P > 0.05).</p><p><b>CONCLUSION</b>Serum containing Semen Arecae, Dandelion, Semen raphani and Atractylodes macrocephala can make notable contraction on colonic smooth muscle cells in rats.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Medicamentos Herbarios Chinos
/
Células Cultivadas
/
Colon
/
Biología Celular
/
Miocitos del Músculo Liso
/
Medicina Tradicional China
/
Contracción Muscular
/
Músculo Liso
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2009
Tipo del documento:
Artículo
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