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Effects of huoxue injection on the adherence of human monocytes to endothelial cells and expression of vascular cell adhesion molecules / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 238-241, 2009.
Artículo en Chino | WPRIM | ID: wpr-273699
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Huoxue Injection (HXI, a Chinese herbal preparation consisted of red sage, chuanxiong, safflower and red peony root) on the expressions of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), and the adherence of monocytes to endothelial cells in cultured human umbilical vein endothelial cells (HUVECs) injured by oxidized low-density lipoprotein (ox-LDL).</p><p><b>METHODS</b>Model of injured cell was established by adding ox-LDL into the culture of HUVECs, and the model cells were intervened with HXI. The adhesive percentage of the model cells to monocytes was determined by protein quantification; mRNA and protein expressions of ICAM-1 and VCAM-1 were determined by RT-PCR and flow cytometry respectively.</p><p><b>RESULTS</b>After HUVEC being treated with ox-LDL for 12 h and 24 h, its adhesion rate to monocytes increased, with the mRNA and protein expressions of ICAM-1 and VCAM-1 in HUVEC enhanced significantly, showing significant differences as compared with those in the normal control (P < 0.05 or P < 0.01). HXI could significantly reverse the above-mentioned changes dose-dependently, showing that these parameters in the HXI intervened cells significantly different to those in the untreated model cells respectively.</p><p><b>CONCLUSION</b>HXI could inhibit the adherence of endothelial cells to monocytes by way of down-regulating the endothelial superficial adhesion molecules, so as to display its protection on endothelial cells, which should be helpful for reducing or suppressing the formation of atherosclerosis.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Venas Umbilicales / ARN Mensajero / Medicamentos Herbarios Chinos / Monocitos / Regulación hacia Abajo / Adhesión Celular / Células Cultivadas / Molécula 1 de Adhesión Intercelular / Molécula 1 de Adhesión Celular Vascular Límite: Humanos Idioma: Chino Revista: Chinese Journal of Integrated Traditional and Western Medicine Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Venas Umbilicales / ARN Mensajero / Medicamentos Herbarios Chinos / Monocitos / Regulación hacia Abajo / Adhesión Celular / Células Cultivadas / Molécula 1 de Adhesión Intercelular / Molécula 1 de Adhesión Celular Vascular Límite: Humanos Idioma: Chino Revista: Chinese Journal of Integrated Traditional and Western Medicine Año: 2009 Tipo del documento: Artículo