Expression of miR-140-5p and prediction of its target gene in human mesenchymal stem cells during adipogenic differentiation / 南方医科大学学报
Journal of Southern Medical University
;
(12): 199-203, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-273788
ABSTRACT
<p><b>OBJECTIVE</b>To screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation.</p><p><b>METHODS</b>Cultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14, and 21 days of induction, the cells were examined for miRNA and mRNA expression profiles using miRNA chip and transcriptome sequencing (RNA-seq) techniques. Correlation analysis was carried out for the miRNAs and mRNAs of potential interest. The databases including TargetScan, PicTar and miRanda were used to predict the target genes of the differentially expressed miRNA.</p><p><b>RESULTS</b>The expression of miR-140-5p was down-regulated and leukemia inhibitory factor receptor (LIFR) expression increased progressively during adipogenic differentiation of hMSCs, showing a negative correlation between them. Target gene prediction using the 3 databases identified LIFR as the target gene of miR-140-5p.</p><p><b>CONCLUSION</b>miRNA-140-5p may play an important role by regulating its target gene LIFR during adipogenic differentiation of hMSCs.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
ARN Mensajero
/
Regulación hacia Abajo
/
Diferenciación Celular
/
Células Cultivadas
/
Adipocitos
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Biología Celular
/
MicroARNs
/
Adipogénesis
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2016
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS