Your browser doesn't support javascript.
loading
Construction of rat bowel transforming growth factor beta1 recombinant adenovirus vector / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery ; (12): 76-79, 2008.
Artículo en Chino | WPRIM | ID: wpr-273883
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant adenovirus vector which regulates the expression of rat transforming growth factor beta (TGF-beta1).</p><p><b>METHODS</b>Total RNA was extracted from F344/N rat small intestine pre-treated with Con A to clone TGF-beta1. pTRE-shuttle vector was used as mediator to ligate TGF-beta1 gene and backbone of replication-incompetent adenoviral vector. The constructed recombinant adenovirus contained tetracycline-responsive element which could regulate the expression of inserted genes. After identification, the desired recombinant adenovirus was packaged in HEK 293 cells. Supernatant of high titer adenovirus was collected to detect the TGF-beta1 gene expression by green fluorescent protein(GFP).</p><p><b>RESULTS</b>The constructed recombinant adenovirus was identified by restriction endonucleases cutting, sequencing, PCR and GFP examination.</p><p><b>CONCLUSION</b>Rat TGF-beta1 recombinant adenovirus is established successfully, which provides material and evidence for further research of dendritic cell (DC) modified by TGF-beta1 to induce immune tolerance in rat heterotopic small bowel transplantation.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Recombinación Genética / Células Dendríticas / Transfección / Expresión Génica / Células Cultivadas / Adenoviridae / Clonación Molecular / Factor de Crecimiento Transformador beta1 / Vectores Genéticos / Genética Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Gastrointestinal Surgery Año: 2008 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Recombinación Genética / Células Dendríticas / Transfección / Expresión Génica / Células Cultivadas / Adenoviridae / Clonación Molecular / Factor de Crecimiento Transformador beta1 / Vectores Genéticos / Genética Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Gastrointestinal Surgery Año: 2008 Tipo del documento: Artículo