Herpes simplex virus-thymidine kinase gene transduced into T cell clone in the treatment of ulcerative colitis in rat / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 72-75, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-273884
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of herpes simplex virus-thymidine kinase (HSV-TK) gene transduced into T cell clone on the treatment of ulcerative colitis (UC) in rat.</p><p><b>METHODS</b>The T cell clone transduced with HSV-tk was infused into 27 rats with UC. Changes of stimulation index (SI), CD4(+), CD8(+), IL-13, IL-4 were detected, and pathological changes before and after infusion was compared.</p><p><b>RESULTS</b>In the second and third day after tk+ T clone infusion, the inflammation of colon was assimilated. Two weeks later, the colon began to renovate and mend. The average SI was 7.39+/-1.24 before infusion, and 2.67+/-0.87 after infusion (P<0.05). Peripheral blood levels of CD4(+), CD8(+) or IL-13 and IL-4 in therapeutic group were significantly decreased as compared to control group (P<0.05).</p><p><b>CONCLUSION</b>HSV-tk gene transduced into T lymphocyte clone and infused back is effective for UC gene therapy and target design in rat.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Terapéutica
/
Timidina Quinasa
/
Proteínas Virales
/
Linfocitos T
/
Terapia Genética
/
Colitis Ulcerosa
/
Ratas Wistar
/
Simplexvirus
/
Genes Transgénicos Suicidas
/
Genética
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2008
Tipo del documento:
Artículo
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