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Cellular sources of interleukin 16 in benign and malignant pleural effusions / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 4160-4165, 2011.
Artículo en Inglés | WPRIM | ID: wpr-273903
ABSTRACT
<p><b>BACKGROUND</b>Interleukin 16 (IL-16) can be detected by ELISA in pleural effusion (PE) and its concentration is higher than in serum. This study investigated the cellular sources of IL-16 in PE.</p><p><b>METHODS</b>The samples of PE were collected from 34 patients who were newly diagnosed having PE in the pleural cavity. We performed cell culture to purify the pleural mesothelial cells (PMC), Wright staining to count the purity and immunocytochemical stain to identify the cultured cells. The intracellular IL-16 expression was detected by flow cytometry (FCM). The different cells in PE were first separated by magnetic cell sorting (MCAS) then the separated cells were cultured in RPMI1640 with 10% fetal calf serum (FCS). We extracted the supernatant and detected IL-16 concentration by ELISA. The IL-16 protein was detected by immunohistochemistry and double immunofluorescence staining.</p><p><b>RESULTS</b>The percentages of cells which secreted IL-16 were CD3(+)CD8(-) cells ((74.27 ± 15.56)%, n = 34); CD3(+)CD8(+) cells ((69.86 ± 18.55)%, n = 34); CD19(+) cells ((45.30 ± 18.77)%, n = 15); CD14(+) cells ((16.91 ± 16.69)%, n = 15); and PMC ((2.05 ± 1.85)%, n = 7). The concentrations of IL-16 in the supernatant from cultured cells were CD4(+) cells ((102.50 ± 42.51) ng/L, n = 5); CD8(+) cells ((92.58 ± 18.34) ng/L, n = 5); CD19(+) cells ((79.85 ± 5.62) ng/L, n = 5); CD14(+) cells ((58.51 ± 25.38) ng/L, n = 5); and PMC ((18.14 ± 8.37) ng/L, n = 5). In lymphocytes, monocytes/macrophages and PMC, we could observe the cells that expressed IL-16 protein. In paraffin-embedded sections, we also could observe by immunohistochemistry the CD4(+)IL-16(+) cells, CD8(+)IL-16(+) cells, CD19(+)IL-16(+) cells, and CD14(+)IL-16(+) cells.</p><p><b>CONCLUSIONS</b>IL-16 in PE is mainly secreted by T lymphocytes, including CD3(+)CD8(-) cells and CD3(+)CD8(+) cells. CD19(+) cells and CD14(+) cells can also secrete IL-16, but the percentage of PMC that can secrete IL-16 is very low.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ensayo de Inmunoadsorción Enzimática / Inmunohistoquímica / Linfocitos T / Linfocitos T CD4-Positivos / Células Cultivadas / Centrifugación por Gradiente de Densidad / Derrame Pleural Maligno / Linfocitos T CD8-positivos / Antígenos CD19 / Receptores de Lipopolisacáridos Tipo de estudio: Estudio pronóstico Límite: Adulto / Anciano / Aged80 / Femenino / Humanos / Masculino Idioma: Inglés Revista: Chinese Medical Journal Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ensayo de Inmunoadsorción Enzimática / Inmunohistoquímica / Linfocitos T / Linfocitos T CD4-Positivos / Células Cultivadas / Centrifugación por Gradiente de Densidad / Derrame Pleural Maligno / Linfocitos T CD8-positivos / Antígenos CD19 / Receptores de Lipopolisacáridos Tipo de estudio: Estudio pronóstico Límite: Adulto / Anciano / Aged80 / Femenino / Humanos / Masculino Idioma: Inglés Revista: Chinese Medical Journal Año: 2011 Tipo del documento: Artículo