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Inhibition of Bcl-2 enhances the efficacy of epirubicin chemotherapy in PC-3 prostate cancer cells / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 4018-4021, 2011.
Artículo en Inglés | WPRIM | ID: wpr-273933
ABSTRACT
<p><b>BACKGROUND</b>Overexpression of Bcl-2 protein in cancer cells can inhibit programmed cell death and engender chemoresistance. Bcl-2 antisense oligonucleotide (G3139) has shown its antitumor effects enhanced in preclinical models when combined with taxol-based chemotherapy. This study aimed to investigate the efficacy of G3139 combined with epirubicin in the androgen-independent prostate cancer.</p><p><b>METHODS</b>PC3 prostate cancer cell line was cultured and treated with epirubicin and Bcl-2 antisense oligonucleotide alone or in combination. The effects of therapeutic agents on cells were determined by the MTT assay. Expression of Bcl-2 mRNA and protein was documented by RT-PCR and Western blotting. Apoptosis induction was confirmed by flow cytometric analysis.</p><p><b>RESULTS</b>Bcl-2 antisense oligonucleotide alone produced no cytotoxic effects and the combination of Bcl-2 antisense oligonucleotide with epirubicin sensitized PC-3 cells to the killing effects of chemotherapy. A marked down-regulation of Bcl-2 mRNA and protein was observed after antisense and epirubicin cotreatment. A statistically significantly higher fraction of apoptotic cells was detected by flow-cytometric analysis after epirubicin treatment with prior antisense Bcl-2 transfenction, as compared with mono antisense Bcl-2 or epirubicin treatment.</p><p><b>CONCLUSION</b>These data suggested that inhibition of Bcl-2 expression combined with epirubicin may be an attractive therapeutic strategy in hormone-refractory prostate cancer.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Neoplasias de la Próstata / Epirrubicina / Supervivencia Celular / Western Blotting / Oligonucleótidos Antisentido / Apoptosis / Proteínas Proto-Oncogénicas c-bcl-2 / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Línea Celular Tumoral Límite: Humanos / Masculino Idioma: Inglés Revista: Chinese Medical Journal Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Neoplasias de la Próstata / Epirrubicina / Supervivencia Celular / Western Blotting / Oligonucleótidos Antisentido / Apoptosis / Proteínas Proto-Oncogénicas c-bcl-2 / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Línea Celular Tumoral Límite: Humanos / Masculino Idioma: Inglés Revista: Chinese Medical Journal Año: 2011 Tipo del documento: Artículo