Study on mechanism of ginsenoside Rg1-induced human neural stem cells differentiation by genechip / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 515-519, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-274313
ABSTRACT
<p><b>OBJECTIVE</b>The molecular targets of ginsenoside Rg1-induced neural stem cells (NSCs) differentiation were screened by genechip.</p><p><b>METHOD</b>7th day following ginsenoside Rg1 induced human neural stem cells to neurons the gene expression was observed by genechip. The purpose gene and signal transduction pathways were selected by the data calculations, and then confirmed by western blot and immunohistochemical method.</p><p><b>RESULT</b>7th day following Rg1-induced NSCs differentiation, there were about 675 different genes, 255 genes of which were up-regulated and 420 genes down-regulated obviously. Meanwhile the ERK1/2 (extracellular signal-regulated protein kinase) in MAPK (mitogen-activated protein kinase) pathway was related with the NSCs differentiation. The Western blot and immunohistochemistry detection confirmed that ERK 1/2 protein and its phosphorylation were significantly increased, which can be blocked by PD98059 (ERK1/2 inhibitor). In addition, differentiation rate of NSCs was also decreased obviously in ginsenoside Rg1-induced differentiated NSCs when ERK blocker PD98059 was used.</p><p><b>CONCLUSION</b>ERK1/2 is an important molecular target in ginsenoside Rg1-induced NSC differentiation. The selected differentially expressed genes by genechip may provide new clues to study of ginsenoside Rg1-induced NSCs differentiation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Factores de Tiempo
/
Flavonoides
/
Regulación hacia Abajo
/
Diferenciación Celular
/
Línea Celular
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Sistema de Señalización de MAP Quinasas
/
Biología Celular
/
Ginsenósidos
Límite:
Humanos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2012
Tipo del documento:
Artículo
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