Study on plant regeneration from somatic embryos of vulnerable medicinal plant Glehnia littoralis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 434-437, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-274329
ABSTRACT
<p><b>OBJECTIVE</b>To study the cause of the seeds dormancy of Glehnia littoralis in vitro and to establish plant regeneration methods via somatic embryos.</p><p><b>METHOD</b>The effects of endosperm and exogenous hormone on the seed dormancy breaking of G. littoralis and the effect of hormone concentration on embryonic callus induction and plant regeneration via somatic embryos were observed,</p><p><b>RESULTS</b>The germination rate of the seeds with 1/3 endosperm was the highest which achieved 31%. TDZ, 6-BA and GA3 treatment could not break seed dormancy but easily lead to abnormal seedlings. Embryogenic callus induction rates was up to 57% on MS supplemented with 1.0 mg x L(-1) 2,4-D. After 20 days culture, embryogenic calli were transferred to MS medium and cotyledonary embryos were formed in 40 days. The regenerated plants were obtained in 20 days.</p><p><b>CONCLUSION</b>An effective system of plant regeneration of G. littoralis was established in this study.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Plantas Medicinales
/
Regeneración
/
Semillas
/
Especies en Peligro de Extinción
/
Apiaceae
/
Técnicas de Embriogénesis Somática de Plantas
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2012
Tipo del documento:
Artículo
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