Phamacokinetic study of bergapten in rats plasma by LC-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 381-383, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-274338
ABSTRACT
<p><b>OBJECTIVE</b>To determine bergapten's concentration in plasma and observe its pharmacokinetics in rats using a combined LC-MS/MS analytical method.</p><p><b>METHOD</b>Blood samples were separated on a Hypersil ODS column (4.6 mm x 250 mm, 5 mm) at a temperature of 30 degrees C, and mobile phase consisted of water and methanol (22.5 77.5) at a flow rate of 0.8 mL x min(-1).</p><p><b>RESULT</b>The methodological study showed a good linear relationship of 8.12-162.4 g x L(-1) (r = 0.9999). The inner and inter-days relative standard deviations were both less than 10% , indicating legitimate precise and accuracy to the requirement of biological sample analysis.</p><p><b>CONCLUSION</b>The method is suitable for in vivo quantitative analysis for bergapten due to its accuracy, sensitivity and specificity. The pharmacokinetic process in rats forms a two-compartment model with first-order absorption.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Valores de Referencia
/
Factores de Tiempo
/
Sangre
/
Farmacocinética
/
Reproducibilidad de los Resultados
/
Cromatografía Liquida
/
Sensibilidad y Especificidad
/
Ratas Sprague-Dawley
/
Espectrometría de Masas en Tándem
/
Metoxaleno
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2012
Tipo del documento:
Artículo
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