UPLC simultaneous determination of five active components in Cinnamomi Ramulus / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3298-3301, 2011.
Article
en Zh
| WPRIM
| ID: wpr-274381
Biblioteca responsable:
WPRO
ABSTRACT
A rapid UPLC method for simultaneous determination of protocatechuic acid, coumarin, cinnamic alcohol, cinnamic acid and cinnamaldehyde in Cinnamomi Ramulus was established. The optimal conditions of separation and detection were achieved on an HSS T3 Column (2.1 mm x 100 mm, 1.8 microm) eluted with a gradient of acetonitrile and 0.05% aqueous phosphoric acid, at a flow rate of 0.5 mL x min(-1), detected at 254 nm. The linear response ranges of protocatechuic acid, coumarin, cinnamic alcohol, cinnamic acid and cinnamaldehyde were 0.359-3.59 mg x L(-1) (r = 0. 999 3), 2. 834-28.34 mg x L(-1) (r = 0.999 8), 0.574-5.74 mg x L(-1) (r = 0.999 8), 2.400-24.00 mg x L(-1) (r = 0.999 9), 32.57-325.7 mg x L(-1) (r = 0.999 8), respectively (n = 6). The mean recoveries (n = 9) of the five components were 96.7%-101.0%, RSD < 2.3%. The assay demonstrates that the method has adequate accuracy and selectivity to measure the concentration of protocatechuic acid, coumarin, cinnamic alcohol, cinnamic acid and cinnamaldehyde in Cinnamomi Ramulus.
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Índice:
WPRIM
Asunto principal:
Acroleína
/
Medicamentos Herbarios Chinos
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Química
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Cromatografía Liquida
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Cinamatos
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Propanoles
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Cumarinas
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Cinnamomum
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Hidroxibenzoatos
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Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2011
Tipo del documento:
Article