Effect of radiation on the activity and expression of Ca(2+)-Mg(2+)-ATPase in rat masseter muscle / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 479-482, 2009.
Article
en Zh
| WPRIM
| ID: wpr-274547
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the activity and expression of Ca(2+)-Mg(2+)-ATPase in irradiated rat masseter muscle.</p><p><b>METHODS</b>The rats were irradiated locally with a single dose of 20 Gy X-ray. The activities of Ca(2+)-Mg(2+)-ATPase were measured with colorimetric method. The protein expression of Ca(2+)-Mg(2+)-ATPase was determined by Western blotting and immunohistochemistry.</p><p><b>RESULTS</b>The activities of Ca(2+)-Mg(2+)-ATPase in masseter muscle decreased by approximately 20% and 40% in irradiated rats on days 3 and 30 postirradiation. There was significant difference in the expression of Ca(2+)-Mg(2+)-ATPase protein between irradiated and nonirradiated rats on day 30 postirradiation. Ca(2+)-Mg(2+)-ATPase protein was found in the cytoplasm of masseter muscle.</p><p><b>CONCLUSIONS</b>The decrease of ATPase activity played an important role in the cause of radiation-induced skeletal muscle injury, while there was no significant reduction in the expression of Ca(2+)-Mg(2+)-ATPase protein in irradiated rat masseter muscle.</p>
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Índice:
WPRIM
Asunto principal:
Efectos de la Radiación
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Traumatismos Experimentales por Radiación
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Inmunohistoquímica
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Western Blotting
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ATPasa de Ca(2/) y Mg(2/)
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Citoplasma
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Músculo Masetero
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Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Año:
2009
Tipo del documento:
Article