Cloning and bioinformatic analysis of FatB genes in Lonicera japonica Thunb and its substitutes / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1394-1398, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-274648
ABSTRACT
A FatB unigene was obtained from the transcriptome dataset of Lonicera japonica Thunb. Full-length FatB cDNA was cloned from buds of Lonicera japonica Thunb., Lonicera japonica Thunb. var. chinensis (Wats.) Bak., Lonicera hypoglauca Miq. and Lonicera dasystyla Rehd. using RT-PCR technology, and named as LJFatB, LHFatB, LJCFatB and LDFatB. The results of bioinformatic analysis showed that LJFatB, LJCFatB, LHFatB and LDFatB and Arabidopsis thaliana AtFatB had a closely relationship. Nucleotide sequences and protein secondary structure of LJFatB, LJCFatB, LHFatB and LDFatB are different and their proteins had conserved FatB substrate binding sites and catalytic activity sites. Transcriptive level of LJFatB, LJCFatB, LHFatB and LDFatB in bud was not significantly different. Therefore, LJFatB, LJCFatB, LHFatB and LDFatB could have the same biological function as AtFatB.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Proteínas de Plantas
/
Tioléster Hidrolasas
/
Química
/
Sistemas de Lectura Abierta
/
Secuencia de Aminoácidos
/
Clasificación
/
Clonación Molecular
/
Estructura Secundaria de Proteína
/
ADN Complementario
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2012
Tipo del documento:
Artículo
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