In vitro experimental study of rat cardiomyocyte injury with targeting of perfluorocarbon lipid particles / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1170-1174, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-274932
ABSTRACT
The present study was to investigate in vitro the rat cardiomyocyte injury with targeting of home-made perfluorocarbon lipid particles with avidin-biotin interaction. Neonatal rat cardiomyocytes were cultured in vitro and divided into two groups TNF-alpha activated group and non-activated group. Those in the TNF-alpha activated group were exposed to 200 ng/ml TNF-alpha solution for 6 hours and then cardiomyocytes in both groups were pretargeted with biotinylated ICAM-1 monoclonal antibodies, and were exposed to streptavidin, and then to homemade green fluorescently-labeled biotinylated perfluorocarbon lipid particles. Cardiomyocytes nucleus stained with Hoechst. The results were detected with fluorescence microscope. As a result, in TNF-alpha activated group, around blue fluorescent cardiomyocytes nucleus, a great amount of green fluorescent particles were found, while there were few green fluorescent particles in non-TNF activated group. It has been shown that ICAM-1 is expressed in the surface of cardiomyocytes when they are stimulated by TNF-alpha. Perfluorocarbon lipid particles associated with ICAM-1 monoclonal antibodies can be targeted to injured cardiomyocytes by avidin-biotin interaction.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Células Cultivadas
/
Química
/
Ultrasonografía
/
Factor de Necrosis Tumoral alfa
/
Ratas Sprague-Dawley
/
Molécula 1 de Adhesión Intercelular
/
Medios de Contraste
/
Miocitos Cardíacos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2011
Tipo del documento:
Artículo
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