Cloning, expression and purification of KDR tyrosine kinase / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1545-1549, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-275324
ABSTRACT
The catalytic domain of KDR kinase (KDR-CD) was amplified from RNA of HUVCEs cells with RT-PCR and expressed in E. coli BL21(DE3) by plasmid pET30a as vector. The recombinant protein was purified with affinity chromatography (Ni-NTA). Western blotting showed that the recombinant KDR-CD was phosphorylated in E. coli BL21(DE3). The recombinant KDR-CD was identified to have kinase activity catalyzing the substrate phosphorylated with ATP in the enzymatic reaction.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Venas Umbilicales
/
Proteínas Recombinantes
/
Clonación Molecular
/
Dominio Catalítico
/
Biología Celular
/
Receptor 2 de Factores de Crecimiento Endotelial Vascular
/
Células Endoteliales
/
Escherichia coli
/
Vectores Genéticos
/
Genética
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2008
Tipo del documento:
Artículo
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