Production and accumulation of xylooligosaccharides with long chains by growing culture and xylanase of a mutant strain of Bacillus pumilus X-6-19 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1221-1227, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-275399
ABSTRACT
Bacillus pumilus X-6-9 isolated from soil and subsequently identified, produced xylooligosaccharides with long chains from xylan and accumulated them in the culture. By improving the culture conditions and mutating the bacterium, a 3.2-fold increase in the production of the xylooligosaccharides was established, when compared to the original culture conditions of B. pumilus X-6-19. The addition of D-glucose to the culture of the mutant strain U-3 of B. pumilus X-6-9 repressed the synthesis of beta-xylosidase, but not xylanase. Thus, it was revealed that strain U-3 was a good organism for the production and accumulation of xylooligosaccharides with long chains from xylan by a microbial culture. Xylanase produced by strain U-3 was purified to homogeneity and characterized. The hydrolyzates generated by the purified xylanase contained xylobiose, xylotriose, xylotetraose, and xylopentaose, but not xylose.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligosacáridos
/
Farmacología
/
Microbiología del Suelo
/
Bacillus
/
Proteínas Recombinantes
/
Química
/
Endo-1,4-beta Xilanasas
/
Técnicas de Cultivo
/
Genética
/
Glucosa
Idioma:
Inglés
Revista:
Chinese Journal of Biotechnology
Año:
2008
Tipo del documento:
Artículo
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