Proteomic Analysis of the Uterosacral Ligament in Postmenopausal Women with and without Pelvic Organ Prolapse / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 3191-3196, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-275538
ABSTRACT
<p><b>BACKGROUND</b>Pelvic organ prolapse (POP) is a major health problem in adult women that involves many factors. No proteomic analysis has been conducted exclusively in POP patients. This study aimed to identify the differential expression of proteins that may be involved in POP by proteomic analysis.</p><p><b>METHODS</b>Samples of the uterosacral ligament (USL) were collected from five POP patients and five non-POP patients matched according to age, parity, and menopausal status and analyzed using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the mRNA expression of proteins that showed differential expression in the proteomic analyses.</p><p><b>RESULTS</b>Proteins differentially expressed between POP and non-POP patients were detected. Eight proteins that were down-regulated in the POP group were identified by MALDI-TOF-MS. These proteins included electron transfer flavoprotein, apolipoprotein A-I, actin, transgelin, cofilin-1, cyclophilin A, myosin, and galectin-1, and their expression was verified by qRT-PCR.</p><p><b>CONCLUSION</b>Using comparative proteomics, we identified eight differentially expressed proteins (including four cytoskeleton proteins and three proteins related to apoptosis) in the USL that may be involved in apoptosis associated with the tissue effects in POP pathophysiology.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sacro
/
Útero
/
Citoesqueleto
/
Actinas
/
Miosinas
/
Apolipoproteína A-I
/
Posmenopausia
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Ciclofilina A
Tipo de estudio:
Estudio pronóstico
Límite:
Anciano
/
Femenino
/
Humanos
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2015
Tipo del documento:
Artículo
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